Can soybean lipoxygenases be real models for mammalian lipoxygenases? A bioinformatics approach

Soybean lipoxygenases (soybean lipoxygenase-1) has been a well-studied enzyme because it is considered as model for mammalian lipoxygenases. In general, the soybean lipoxygenase-1 used in test of inhibitory activities various compounds. The present study provides bioinformatics approach comparison databases. Their physical and chemical parameters such molecular weight, theoretical pI, amino acid composition, aliphatic index grand average hydropathicity multiple sequence alignments were computed by using several tools. order to see phylogenetic relation among lipoxygenases, tree was constructed. first three most abundant acids 15-lipoxygenase (human) are L (10.3 %), S (7.4 A (6.7 %) (13.3 G V (7.1 respectively. According tree, within separate clade compared conclusion, may not fully characterise human lipoxygenase-15 since there remarkable sequence-based differences, which obtained tools between from other sources especially human. this context, researchers aware problems described above having similar concerns have begun use recombinant DNA technology produce h-15-LOX Escherichia coli.